certainly yes!!! The conservationist criterion implies maintaining the genetic pool. The act of self-pollination is to fulfill the role of an insect, and thereby keep the genome of that species intact. Is that what you want?

@ximena thanks for your input. But my mere misconceptions or misunderstanding is, would there be any variants or will it maintain the parental genetic make up intake???

By using selfed seeds you will decrease drastically the genetic variations between the individuals produced compared with the wild ones. Its seedling won't have the same genetic make up with the parent plant or it will be highly unlike due to chromosome crossing overs etc. If you want to have seedling with the same genetic material as the 'father' plants the best option will be by creating clones by dividing the tubers (if the species have) into more pieces, this way you can also avoid genetic abnormalities which can affect the plant development. As for in - vitro seed multiplication aiming for conservation the best way will be from seeds harvested from different population of the species.

@spyridon thanks for you valuable inputs

I agree with Spyridon.

I want to add the following: In a conservation program, it is necessary to have a criterion of inclusion of "everything that is in nature could disappear". This means that the genetic variations that occur naturally, must be conserved too, because they are adaptations of the species to their environments naturally. So, everything you keep is valuable. However, this does not exclude genetically improved material, because it has been obtained by a selection process directed to preserve or highlight some superior character.

Dear Dr. Aheibam Loyanganba Meitei , you have raised a critical issue on one of the sensitive issues of that contributes for saving the depletion of orchids.

Dear Dr. Aheibam Loyanganba Meitei, an effective conservation strategies is very important to prevent further loss of the already depleted orchid population.

The in vitro approach through the application of plant tissue culture technology provides an excellent opportunity for effective conservation by mass propagating orchids in short time span.

For this,

• efficient and reliable protocols for seed germination,
• understanding of culture growth and development in vitro,
• reduction of death rate during hardening process, and
• commercial scale micropropagation techniques for propagation of D. chrysotoxum are expected to be developed properly for its sustainability.