And can we use it for conversion of any of the peptide (in Beta form) to its monomer form?
HFIP is a strong inducer of alpha-helical structure, hence it tends to disrupt beta-structure in short peptides. I can't guarantee that it'll work with all peptides, but it would always be one of the first solvents I would try.
A particularly good property is its low boiling point, i.e. it is easy to remove HFIP via evaporation if it is not needed anymore. This one is not so easy with most of the alternatives (ammonia etc.).
HFIP is a strong inducer of alpha-helical structure, hence it tends to disrupt beta-structure in short peptides. I can't guarantee that it'll work with all peptides, but it would always be one of the first solvents I would try.
A particularly good property is its low boiling point, i.e. it is easy to remove HFIP via evaporation if it is not needed anymore. This one is not so easy with most of the alternatives (ammonia etc.).
To bring peptide to its monomeric form, you can use HFIP:TFA (1:1) to dissolve the stock. As Holger said, this combination is also easy to evaporate. You can add appropriate buffer to the dried film and carry to assays.
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