I performed hemoglobinase activity of my recombinant enzyme expressed in E.coli with partial purification and I'm not sure if the activity comes from my enzyme or impurity from host.
To look for hemoglobinase vs other proteases, you could consider pH and Cys protease inhibitors. Hemoglobinase degradades human hemoglobin at acidic pH (3.0-5.5). It will also be inhibited by cysteine proteinase inhibitor (e.g. E-64).