Hello everyone! I am planning to perform β-Galactosidase staining on plated cells. In order to perform a proper object segmentation of cells/nuclei upon image analysis, I would like to use a nuclear dye. I was thinking about Hematoxylin or DAPI. I know that a successful β-Galactosidase staining depends on proper pH, so I am not sure Hematoxylin is the best nuclear dye in this context.
Thank you in advance
Alessio