How to calculate total flavenoid content?

16 August 2020 5,323 4 View

We are planned to conduct status of communicable disease among health institution in Gondar after COVID 19. what type of study design is appropriate?

anyone who had a tool to assess the above-proposed project please help us?

29 April 2020 1,477 8 View

How to calculate individual fatty acids in egg GC-FID analysis?

Details of how to quantify each fatty acid contribute to the total. Formula and procedures of calculation and interpretations...

01 January 2020 9,170 3 View

Does anyone know a company that manufactures a continuous pancakes, dosa or kisira making machine that can be used as such or tailermade for injera?

I was wondering if there exists a company that manufactures a continuous pancakes, dosa or kisira making machine that can be used as such or tailermade for injera, an Ethiopian sour bread? Please...

11 July 2019 3,209 3 View

Any good suggestion of materials that help learn R stat for beginners?

I have never used R stat before, because of the requirement of knowing programming language. But apparently, it is the only freely available program for statistics. I used SAS a couple of times. I...

17 April 2019 4,262 8 View

Can phosphate-solubilizing microorganisms (PSM) are available on market? is that possible to cultured them in the laboratory and with what media?

Because, P-solubilization ability of microorganisms is considered to be one of the most important traits associated with plant phosphate nutrition

07 April 2019 633 2 View

How to choose the correct POSCAR for VASP calculation?

Hi I have proposed a low energy structure of (ZnS)6 cluster using genetic algorithm software and prepared POSCAR (1 POSCAR) using VESTA for VASP calculation. But the POSCAR I obtained from...

20 March 2019 7,406 3 View

Is there a strut and tie model (STM) independent of the support plate and loading plate dimensions?

The capacity of the strut and node in the STM depends on the dimensions of the support and loading plates; however, these values are not provided in most of the literature.

18 June 2018 8,652 2 View

What do you do on the first day of class in a college or university?

What do you do on the first day of delivering a course in a university or college to take up your semester on the correct path, and operate your first day of a course into the best learning...

24 December 2017 1,935 3 View

How can undergraduate students get to the point where they understand something well?

Memory is one of the fundamental tools in the learning process. However, there is a significant difference between memorizing something and learning it. Then, how can undergraduate students go to...

15 November 2017 5,937 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View