Is CO2 needed during lentivirus spinfection?

14 June 2021 1 995 Report

I'm currently using HEK293T cells. Most spinfection protocols I have found did not state clear about if CO2 is needed during the spinfection in centrifuge after cells and virus are mixed. As 5% CO2, usually from the incubator , is necessary to maintain correct pH, would complete DMEM containing 5% CO2 be needed?

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