Hi all, I am trying to identify NK and NKT cells in mouse splenocytes. I am identifying CD3+CD335+ cells as NKT cells but I am not sure whether CD3-CD335+ would correctly identify NK cells. Any help is appreciated!
CD3+CD335+ cells will be a small fraction compared to CD3+NK1.1+ cells. Regardless both NK1.1 and CD335 will be expressed by a majority of NK cells. CD335 is expressed in NK cells and iNKT but not on CD1d restricted NK cells. For C57BL/6 mice I will use NK1.1, CD335, CD4, CD8 and TCRbeta