Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

Why I see Lamp-1 GFP in cytocol after transfection?

Hi every one I transfected Lamp-1 GFP plasmid to the HEK293 cell line. When I image the cells in TIRF, I see green fluorescence o the cytosol too. Does anyone have any idea that how it can...

21 December 2020 9,978 3 View

Why HEK293 transfected with LAMP-1GFP do not load dextran?

Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...

14 December 2020 1,801 2 View

What are the tools or software which can simulate climate change for a city and conclude future climate data?

I am working in research on climate adaptation so need to compare the performance of the urban fabric to the climate changes, which requires me to make a comparison and simulation of the...

03 December 2020 988 8 View

Correlation, Regression and significance?

Dears, I have different variables in multiple regression. Two variables are highly correlated. To solve the collinearity issue, I dropped one of them from the multiple regression model. The...

29 November 2020 2,695 4 View

Do anyone have any authentic material regarding Cultural Ethics and especially regarding Pakistani culture?

I need this because I have to represent it in a conference as a keynote speaker

17 November 2020 584 4 View

How can I synthesis heparin nano particle?

I want to synthesize iron nanoparticles with heparin, but I have trouble synthesizing First of all, do I have to use a freezer drying for drying of product and it is not impossible use from...

16 November 2020 9,455 2 View

How to differentiate between relationship and effect using statistical terms?

It is easy to understand the meaning of relationship and effect (impact) in general. But when it comes to the statistical analysis it is a difficult conundrum, as one can determine the...

24 July 2020 6,525 7 View

Which is the best solvent for herbal extraction?

We are aware of the fact that some type of solvent is required for isolating a compound from herbs. I personally believe that water is the best solvent since mostly decoction is prepared using...

22 July 2020 4,179 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View