Is anyone using Winepiscope 2.0 epidemiological veterinary medicine software for risk analysis?

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What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

How to construct Scatter plot for KEGG or GO classification visualization?

Can anybody tell me how to construct this scatter plot for KEGG or GO classification like the image I have attached?

27 September 2020 5,359 3 View

Is RUPKATHA Journal on UGC Care List?

I recently presented a paper in the Rupkatha International Online Conference 2020, and I'm all set to submit my final paper for publication. But I just came across a feedback on the page that...

23 August 2020 1,714 7 View

Approximate timeline in months for Systematic Review without meta analysis?

Hello everyone, I along with few of my colleagues are planning to do systematic review on Covid patients with particular sign ,symptoms and clinical implications mentioned in various published...

14 August 2020 8,096 9 View

Any instances of when an antibody will bind better if left at RT for days?

Can anyone make sense of this: an antibody was left out for 7 days on the bench at RT and binds better to a metalloprotein than if the antibody was taken out the day of the experiment?

15 April 2020 6,330 3 View

Is there any "Biostatistics" online course for Animal Science?

Use of proper statistics to analyse research data is the most important thing. Please suggest me some "Practical" biostatistics course which is available online. Thank you.

19 March 2020 1,931 5 View

How can the "r squared" value of regression analysis be interpreted in Nutritional Requirement study?

Researcher use regression analysis (both linear and quadratic) to express how the growth and other relevant parameters correlated with the graded level of nutrient of interest. In a crude way, it...

13 March 2020 7,064 6 View

Why do most researchers use 0.25M sucrose solution for tissue homogenate preparation?

Generally, the 0.25M sucrose solution is the most common organic solution which is used for tissue homogenate preparation. There are many specific buffer solutions which are used for tissue...

28 February 2020 8,470 2 View

How do I calculate solubility of a compound using a turbidity assay?

I'm planning to measure solubility of my compound in PBS buffer by measuring the turbidity of serially diluted solutions in a microplate reader at 600 nm. Can anyone please inform me how to...

23 November 2019 3,333 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View