Is anyone using tetracycline hydrochloride solution for rat bone labeling?

25 February 2014 1 8K Report

I have difficulties in solubilizing tetracycline hydrocloride to label rat bone. The concentration I'm using is 15 mg/ml in physiological saline: doesn't solubilize well. Any suggestion? What if I use distilled water, do I risk to harm the rats?

S.A. Abbah

Have you considered calcein (green), xylenol (orange) and alizarin (red)? These may be better options to tetracycline as they cause less impairment to bone growth and mineralization.

Badges
Science topic

More Francesco Cavani's questions See All

How to determine the charge passing during a cyclic voltammetry?

Dear All, here is my question: if I have a 15-scans cyclic voltammetry performed with PalmSens, how can I obtain the charge passed during the process? Hopefully avoiding integrations,...

29 July 2024 3,733 5 View

Differences between Surface Tension and Interfacial Tension in Liquid-Gas Phases?

Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...

18 July 2024 382 5 View

EIS spectra analysis software?

Dear all, I'm currently working with CHI potentiostat and performing Impedance. However, for the circuit fitting the instrument must be connected to the computer. Since I prefer to analyse the...

10 July 2024 8,880 4 View

How to perform a double step optimization with ORCA?

Hello to everyone, I am trying to launch a BLYP optimization with ORCA starting from an already converged calculation at BP level ,exploiting its orbitals and geometry as a guess. I tried...

24 June 2024 3,768 2 View

Is a binary classifier based on Gaussian models resistant to the problem of training set imbalance?

A binary classifier based on multivariate Gaussian models, which estimates the mean vector and the variance-covariance matrix during the training phase and returns the class with the highest...

23 June 2024 10,114 1 View

Odd structures in transmission electron microscopy?

Dear all, I found in cell cultures some structures that I cannot identify I will really appreciate is someone would give me some suggestions Many thanks Francesco

09 May 2024 2,070 11 View

APOe isoforms Sanger sequencing - good primers and protocol?

Hello everybody. For my project i need to identify the APOe isoforms present on my patient derived lines. My idea was to extract DNA, amplify with PCR and send it for Sanger sequencing -...

24 April 2024 6,359 4 View

Why do we use an oblique rotation in Factor Analysis when data is highly correlated?

What should we do with high-correlation data? Did we remove them, or do they not matter? WHY?

12 April 2024 9,429 3 View

Double-counting of emissions in the context of ISO 14068 - Carbon Neutrality assessement?

Greetings. In the context of the new ISO 14068 on Carbon Neutrality, how is double-counting of emissions between two subjects been dealt with? Suppose Company B is assessing the carbon neutrality...

03 April 2024 3,596 1 View

Do age- and/or sex-specific allometric scaling indexes for muscle mass on body weight exist?

Is there any standardised index to estimate, more or less precisely, muscle mass from body weight (e.g., Bodyweight * index)? Any reading suggestions are much appreciated.

27 February 2024 8,285 1 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View