How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

Can zinc based fixatives be used after enzymatic dissociation of neural tissue?

I am working on a FACS experiment with mouse neural tissue. I want to preserve all cell types as much as possible and the zinc fixation papers I have read use mechanical dissociation which can...

21 January 2021 4,430 1 View

Is there a technique to store fixed cells after flow for later RNA analysis?

I am separating neuron populations from mouse brains using FACS. I am trying to find a fixation technique for intracellular antigen staining and FACS that will allow long term storage afterwards...

21 January 2021 9,722 3 View

Removing vitreous from baby rat eyes?

I have been trying to remove the vitreous humour from baby rat eyes (post natal day 15) and have been still having a lot of interference in my IHC staining. Does anyone have a good protocol to...

20 January 2020 4,634 1 View

Test for silicone residue from silicone part?

I have a silicone part that will come in contact with food. It will need to pass FDA tests for hexane extraction of silicone residues. Is there a method I can use to remove uncured material and...

09 December 2019 4,246 3 View

ANSYS Meshing Issue - How To Mesh Complicated Geometry (~80,000 Faces)?

I am attempting to mesh a complicated design (~80,000 faces) for a microchannel heat sink, as pictured, and I would appreciate some advice. I have tried a range of different mesh controls...

08 October 2019 5,610 7 View

How to get higher concentration of linearised plasmid?

I am Trying to use Infusion cloning to prepare a mutant construct. I have been told by a student that used it before that I need 20ng/ul of linearised plasmid to proceed with the following...

04 April 2019 646 10 View

Who are leading theorists today on memory ?

Specifically memory when writing memoir.

03 April 2018 3,172 2 View

I wish to inactivate mammalian source phospholipase activity, can anyone offer insight or references to published work?

I'm looking to mitigate in vitro phospholipid degradation over extended periods of time.

20 February 2018 8,740 3 View

Are likelihood-based approaches to statistical modelling REALLY removing the arbitrary problem of 95% significance?

It strikes me that likelihood-based approaches are prone to the similar dichotomous decisions as traditional hypothesis testing. E.g. In the environmental sciences we now seem to accept that...

14 October 2017 1,264 8 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View