Hello!
I'm looking for a protocol to prepare Candida antigen extract, in order to perform skin tests. Can anyone give me some advice?
Thanks.
CANDIN® is commonly used for Candida albicans) Skin Test Antigen
For Cellular Hypersensitivity assessment
It is also being studied for HPV intralesional injections
Please review literature on this product in methods
First culture it in liquid media for 8-10 days, and then subject it to sonication ( high speed) in ice. Then make a wet mount and see. If more than 80% cells are lysed, then antigen is ready to use.
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