Is anyone familiar with the protocol for Candida antigen extract preparation?

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In theory, can metabolic diseases, characterized by the lack of production of a protein, be treated (not cured) by supplementing with that protein?

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Amar Safdar

CANDIN® is commonly used for Candida albicans) Skin Test Antigen

For Cellular Hypersensitivity assessment

It is also being studied for HPV intralesional injections

Please review literature on this product in methods

Sayan Bhattacharyya

First culture it in liquid media for 8-10 days, and then subject it to sonication ( high speed) in ice. Then make a wet mount and see. If more than 80% cells are lysed, then antigen is ready to use.