Protocol is about NK cells (CD56 APC / CD16 PE). Flow cytometer is really old, so I want to make the right adjustments. The signal options I can change in the protocol, are only FS & SS, FL1-FL4 and AUX. Main aim is to recognize specifically NK Cells (isolated from PBMCs) and distinct their different subpopulations. (CD3+ cells gated out)
Thank you!
Hello Vasileia, What instrument do you have? There should be little spill over between PE and APC to worry about - so set voltages for unstained control in the first log decade(left hand side) - if possible, you want to be able to see both sides of the population curve.
Check that the single stained control(s) intensity is on the linear scale(VISIBLE on the right hand side of the axis). Compensation spill expected FITC -> pe -> PerCP ->APC for balanced parameter voltages. Best wishes
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