Rose Bengal is not for preservation but for staining the live cells that were well-conservated for formol for instance.
Dear Lília P. Souza-Santos, you are right.
Actually I just wanted to know is there any second option for staining purpose?
Hi,
For sorting purposes Rose Bengal is the best, but depends what size fraction you have. For identification, you could try a Methyl Blue stain.
Now there is also the CellTracker Green CMFDA (5-chloromethylfluorescein diacetate) .
See this paper : http://www.biogeosciences.net/10/4565/2013/bg-10-4565-2013.html
Erythrosin can be used as an alternative to Bengal Rose. Both are chemically related. It has about the same effect on fauna but it often stain less vegetal cells.
Cannot add valuable "staining information" here but would like to point to another thread in RG with "Rose Bengal" from July 2014:
https://www.researchgate.net/post/Can_anyone_explain_why_a_rose_bengal_stain_might_not_work_on_shallow_marine_benthic_foraminifera
perhaps there you will find some valuable information. More you can find using the Search Function of RG (see upper Menue bar, right to JOBS find the symbol "PERSON" which is attributed to "search Researchers", klick into and find the pull down menue, klick QUESTIONS and insert as search phrase/word(S) e.g. rose_bengal
(this creates the URL: https://www.researchgate.net/search.Search.html?type=question&query=Rose+Bengal and you'll find a lot of Questions and Answers regarding ) Best wishes and good luck
As Frédéric points out, erythrosin is a good alternative for rose bengal. It is also called eosin red, and is soluble in water so is quite safe. It is also relatively inexpensive. We have had good results using eosin for picking benthic samples from a range of environments.
From the abstract of:
Williams, D.D. & N.E. Williams 1974. A counterstaining technique for use in sorting benthic samples. Limnol. Oceanogr. 19 (1) 152-154.
The primary stain, rose bengal or Lugol’s iodine, is counterstained with chlorazol black to provide a high color contrast between the animal and detritus fractions of such samples.
What's your intended purpose? Rose Bengal works well for sorting small invertebrates. Lugol's is a bit harsh, but it can be helpful on subsets of material. Not sure if Congo Red has been used for sorting inverts.
Dear all, I think this is a very relevant issue.
I agree that Bengal Rose is the best choice for sorting but, as far as I'm concerned, a lot of chemical companies are not selling Bengal Rose any more, they just offer some products (for microbiology) which include Bengal Rose. Given that fact, unless we find some Bengal Rose supplier, we really need to find an alternative for staining with sorting purposes. Fon identification, nothing like Methyl Blue.
Warm regards!
As long as the color index matches, then the name (Rose Bengal or Bengal Rose) won't matter: C.I. 45440. I haven't tried methyl blue. Methyl green might be another good one.
Dear all, just to inform:
SIGMA-ALDRICH (at least in AUSTRIA&GERMANY, cf.
http://www.sigmaaldrich.com/catalog/product/aldrich/330000?lang=de®ion=AT&gclid=CNjwp6y-y8UCFQHJtAodTnIAfQ
offers
# 330000 Aldrich Rose bengal
Dye content 95 %
Synonym: 4,5,6,7- Tetrachloro- 2′,4′,5′,7′-tetraiodofluorescein disodium salt, Acid Red 94, Bengal Rose B sodium salt, Rose Bengal sodium salt;
Packaging:
1, 5 g in glass bottle = # 330000-1G [€ 62.50]
5 x 1, 5 g= # 330000-5G [€ 250.00]
CAS Number 632-69-9
Empirical Formula (Hill Notation) C20H2Cl4I4Na2O5
Molecular Weight 1017.64
Beilstein Registry Number 3645857
EC Number 211-183-3
MDL number MFCD00005043
PubChem Substance ID 24859827
This said (information copied and pasted from the cited URL) follows Disclaimer: No financial interest, the stuff is or might be provided and/or sold also by several other companies (cf. http://www.chemexper.com/chemicals/supplier/cas/632-69-9.html)
best wishes and regards,
Wolfgang
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