Is ammonium sulphate interfere DNS assay ?

12 December 2017 1 9K Report

I did ammonium sulphate precipitation for my enzyme but it failed to give DNS test. While the same precipitate gave positive result in agar test I.e. give clear zone in pullulan agar medium. Is ammonium sulphate inhabiting the DNS assay or something else? Please suggest.

Koteshwara Ananthamurthy

Yes it does. Dialyze your protein solution before performing any test.

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