Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm whether it is detrimental or not to align both types of sequences.
For some context I have collected 87 sequences from a frog family. Some of these species are fairly unknown so the choices for DNA sequences are limited.
I have got RAG1, Cyst and 16S rRNA sequences so far and my tree looks okay (as far as I'm aware).
Thanks:)
Article Sequence analysis of the complete mitochondrial DNA in 10 co...
Maybe you could use Partitionfinder (https://www.robertlanfear.com/partitionfinder/) to test if it's ok to run your analysis as a unique sequence or if it would be better to partition it based on different substitution rates. Some algorithms (like the one on RAxML) can construct a tree based on partitioned data with reliable results.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology