Using OpenFoam, which solver is the best to describe the hydraulics of Wastewater Stabilisation Ponds?

Hello everybody. I would like to receive some tips to make a good CFD modelling of the Hydraulics of Wastewater Stabilisation Ponds. Please, I need an orientation about that. Thank you in advance!

02 April 2018 6,251 3 View

What is the best non-expensive conventional protocol for green microalgae DNA extraction??

I've been trying to isolate DNA from Dunaliella microalgae using the plant DNAzol protocol without been successful yet. The yield is quite low with too poor quality DNA. I observed that after...

26 July 2017 4,474 4 View

What is happening with qpcr using Evagreen?

Hello, in my lab, we just start to work with Evagreen.The same samples wich amplify the constitutive gene (HPRT) with specific probe in a 24 CP, using Evagreen this amplifies in a 38 CP...this is...

04 May 2017 3,275 3 View

Does anybody know of any work on decision trees with multivariate response?

Basically, I am looking for help on how to perform classification and regression trees when you response variable is more than one dimension. Any help on R implementation or any comments on how to...

01 May 2017 1,018 2 View

Does anyone know how to analyze ELISA assays using R?

I need to know how to apply NSB and B0 wells ran in duplicate. How do you fit duplicate standards to a 4/5 parameter logarithmic curve and then interpolate using %B/B0? The idea seems straight...

04 March 2015 4,507 1 View

Can you help solve this problem with JC-1 (Mitochondria membrane potential dye) staining?

I have been attempting to stain LN229 glioblastoma and MSU1.1 human fibroblast cells with the mitochondrial membrane potential dye (JC-1). The dye localizes to mitochondria using the potential...

05 April 2014 9,205 7 View

Is a natural phthalate possible, or is it just a contamination?

I have seen in some papers the report of "natural" compounds with phthalate structure. I think that is just contamination

25 December 2013 9,953 3 View

Is there a method to determine TiNi nanoparticles?

I need to determine (quantitative) TiNi nanoparticles. They are in aqueous solution, but the problem with the synthesis is that (probably) there are TiO2 or Ti and Ni metals in the solution.

14 February 2013 7,815 4 View

Any tips for keeping a 'not-so-clean' culture room ~sterile?

01 January 1970 4,964 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View