Dear Researches
I have studied on the interactions between safranal, a constituent of saffron, and micrtotubule protein extracted from sheep brain. I could prove anti-tubulin activity of safranal in vitro which make safranal as a novel natural agent to be useful in cancer therapy. Unfortunately, some experiments are required for publication of this study and the relative instruments are not available for me now.
Kindly, It is my pleasure to invite you officially to contribute at this project. The manuscript are ready and some experiments are missed. So, I don't think these required experiments take more than one month. I'd like to inform you that your name will be at third place in the paper.
Please feel free to contact me for more details.
Ali Dadras
Institute of Biochemistry and Biophysics
Hello Ali,
What contribution is needed? My first degree is in Biology & Biochemistry.
Dr. Miranda Yeoh
Selangor Matriculation College
Ministry of Education, Malaysia.
It sounds interesting,
please tell me how you see those experiments!!!
I appreciate the invitation to contribute for your project. Please, let me know what experiments are needed?
Hello,
Tell me more about the experiments? Which determination you need?
Dear Researches
I appreciate for your willingness. If you have adequate facilities, I need Tubulin to extracted and titrated by various concentrations of safranal so that we can analyze safranal binding sites experimentally through fluorometry method. I have carried out the computational analysis but it is needed that our data to be supported by fluorescence experiments. By the way, we need to examine safranal effects on some cancerous cell lines (e.g. PC12).
If you can do these up to 1 or 2 months, I would be grateful. I must emphasize again that your name will be at third place in this paper and if you introduce your colleagues, their names will be placed at this paper as contributors. I will send you any documents you need and help you to do the experiment design.
We can publish this novel study in high reputation journals with your kind assists. The manuscript was written and I will send you before submission. It will take about 2-3 weeks.
I am looking forward to hearing from you at your earliest convenience.
Sincerely
Ali Dadras
Institute of Biochemistry and Biophysics
Dear Ali,
Which sorts of fluorescence experiments (wide pannel) you want to use : optical microscopy, confocal analysis ... ? and to obtain which data: colocalisation of your product + cellular organels ie.
I think, you have to build your experimental plan and to determine which question you want to solve in complement with your personal data; then , you could choose the most appropriate florescence experiments, and the best technic to answer your question. And colleagues who have the technology could help you.
Kindly
Didier JAMBOU
Dear Didier
I have a complete plan for the experiments needed. I have designed experimental plans step by step. I actually don't have any questions and I'd like to find experienced persons to carry out such experiments.
If somebody is found as candidate for this issue, I will send the all documents needed.
Best Regards
I didn't want to be unkind !
Just if you have no questions, so why do you want to complete your experiments ? and by fluorescent experiements ? you could publish directly ?
Now, If flurorescence experiments are usefull to confirm your data,, the problem is that it's a "generic" term , meaning that you use a fluorochrome as labelling marker; and you have a lot of different technics using fluorescence labelling , and the specialists using them are different.
No body is able to perform all the technics using flurorescence (and have the corresponding skills).
Some non limitated examples: you have ELISA technics, for all compounds (prot, DNA ...), microscopy with optical detection, or FISH, or confocal one (colocalisations)... quantitative PCR analyzers, genotyping by Sequenom ... etc
I didn't want you to detail your experiments , but to know if I am a specialist as you want, your request is not sufficiently precise.
So, you can see my CV and the fields "with flurorescence" for which I have skills.
Over advices of colleagues ?
Regards
Didier
PS "candidate for this issue" is a strange term to adress to "experienced" colleagues, who perhaps don't need especially to publish, but who are like me: always open mind to collaborate with all researchers in the world, of all levels.
- Badges
- Science topic
- Similar topics
- Filing