Hello
Recently I worked about in vivo drug efficacy test with back flank subcutaneous cancer cell line xenograft model.
I injected intratumorally with drug diluted in high-glucose (With 4500 mg/L D-glucose) media.
But with or without the drug in the media, some mouse were dead just after intratumoral injection.
These mouse were dead 1min~30min after injection, and they show a little paroxysm symptom and body temperature is fastly decreased.
When I did the autopsy just after mouse dead, there's no media that I injected nearby the tumor site, and even if intraperitoneal area. So I guess the media that injected were fastly absorbed by tumor angiogenic vessel and circulate whole body make some shock.
And that shock might be some hyperglycemia, or other unknown shock.
Is there anyone get the similar situation during mouse experiment?
Hi Dr Hong,
First of all, the tumor efficacy experiment recommends that you can use inactivated PBS as a primer. Here are a few possible causes of death in mice: 1. In the case of high sugar, the mice's blood sugar imbalance can cause many diabetes complications, burden the cardiovascular system, and cause death. Verification Method: The control group was arranged to inject only high-sugar base solution. You can look at the survival of the control group. 2. In situ injection causes the capillary to rupture. Previously, I usually used intravenous methods for drug efficacy experiments (more closely related to clinical use). 3. The drug has adhesion, if the molecular weight of the drug is too large, it will cause a certain cardiovascular burden, you can try multiple, less quantitative injection methods. Recommendation: Gradient test of injection volume can be done. Find the cause of death. If you have any questions about the mouse experiment, please feel free to contact me. It is a pleasure to discuss and share your experience with you.
Please connect me feel free: [email protected]
Jason Fang Ph.D
Asia BD
Gempharmatech CO., LTD.
It’s not good enough to cite some vague effect such as “cardiovascular burden” or “sugar imbalance.” You must first describe a testable mechanism that explains specific effects, and the more specific the better. Real science is extremely difficult. Sloppy science that relies on statistics is useless. At best it might provide some vague clue that something is happening, but it is just as likely to be misleading.
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