No smears on gel after cDNA synthesis ?

Hi all, I have a problem with detection of cDNA synthesis where when I put it on the gel , there were any smears so what the meaning of that and what can I do? Thanks

21 December 2020 1,126 2 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

How to develop a framework/model and present it in an analytical form?

Dear All, I am conducting a study on DBD actuators and I'm trying to develop a framework for actuators using plasma-based metamaterials. In order to reach this objective, I need to learn how to...

01 June 2020 3,864 2 View

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange?

The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...

10 May 2020 7,879 3 View

Are ACEIs underdeveloped in children?

COVID19 shows less affection of lung in children . this may be due to underdeveloped or decreased expression of ACEI in lungs of children taking in consideration that as age increase from 5...

28 March 2020 2,448 2 View

Solution not converged at time ........... in ansys?

I am doing four point flexural text on simply supported beam using ANSYS APDL first with steel plate for loading and supporting and then without it. In both cases there is a problem of non...

19 March 2020 4,775 6 View

Can we find the Link between Teaching Methods and Achievement in Math in Computer-Assisted Elementary Schools?

I published a paper in: Creative Education, Published Online http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47205 The Link between Teaching Methods and Achievement in Math in...

14 February 2020 1,408 4 View

How to detrend a series in Stata when it has a stochastic trend?

08 February 2020 1,803 3 View

What is the editorial office email of this journal?

I submitted an article and received an email stating that I need to fix the word count to 5000 or less., though the word count in my manuscript is 4330. I sent back an email in this regard and...

06 October 2019 9,154 3 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Dear all I would like to get some advice from you that I am confused to convert the Cartesian coordinate system to a flow-oriented coordinated system?

Dear all, I would like to get some advice from you that I am confused to convert the Cartesian coordinate system to a flow-oriented coordinated system during XYZ data interpolation?

27 December 2020 695 3 View

How to handle missing values?

Dear colleagues, What can you suggest in order to fill the missing values, as you can see in the picture? Best Ibrahim

24 December 2020 4,834 5 View

ARDL bounds test on Eviews: does multicollinearity impede the model from running?

Hi everyone, I hope I can get some help. My group is running an ARDL bounds model to test the Environmental Kuznets Curve in Cyprus. We're trying to perform it on Eviews, but we can't seem to be...

02 December 2020 9,230 4 View

Difference between ECM Regression and CEC Regression?

What's the difference between the ECM Regression in the ARDL Error Correction Regression and the Conditional Error Correction Regression in the ARDL long run form and bound test as seen in EViews

02 December 2020 6,770 1 View

How to conduct CUSUM and CUSUMsq test after the inclusion of an exogenous dummy variable in my ARDL model?

Hi all, I have been struggling a great deal with this matter and as such I hope you could provide me with answer. I have added a dummy variable to my ARDL model to improve its stability (Eviews)....

01 December 2020 1,071 5 View

I have GLDAS daily satellite data of rainfall for districts. I used SDSM and got monthly mean. In ArcGIS how I map without having districts points?

Actually, the data is satellite data from NASA Giovanni and when I was obaining the data I gave location values in the form of latitude and longitude for the whole area of each district not one...

30 November 2020 7,850 2 View

How to solve spikes and peaks in interpolated raster surfaces with Kriging?

25 November 2020 9,657 4 View

How to run an MGARCH-BEKK MODEL in eviews and interpret the results?

19 November 2020 4,350 3 View

What are the basic to state-of-the-art methods for INDOOR temperature interpolation from sparse measures?

Hello, I'm about to start a new industrial project in which we want to estimate (interpolate) the INDOOR temperature at unknown 3D positions in possibly complex 3D room geometry based on sparse...

28 September 2020 4,137 3 View