At what temperature the enzymes that split nonstructural carbohydrates in plants are killed?

29 October 2020 6,410 4 View

Finger tapping - which sensor?

We are developing a markerless software for the measurement of bradykinesia (finger tapping) using two monochromatic IR cameras and three infrared LEDs. We would like to validate the results with...

19 October 2020 6,819 2 View

Boiling temperature of 50% ethanol at 6000 m a.s.l.?

18 October 2020 7,718 4 View

What kit use for bacterial dna extraction?

Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...

30 July 2020 6,566 7 View

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)?

Was anyone successful in IHC staining of c-Fos or Egr-1 on paraffin embedded sections from mouse olfactory sensory epithelium (VNO, MOE)? Especially, not using Santa-Cruz antibodies? I am being...

04 June 2018 3,394 3 View

Can I detect autophagy via FACS with extracellular staining?

I'm just trying to organize balanced panels to describe lymphocytes from my patients and I was wondering if its possible to detect autophagy with extracellular staining. I suppose Beclin 1, LC3 or...

09 July 2017 1,116 3 View

Hello everybody! Does anyone have experience with isolating bacterial DNA from skin swabs?

23 July 2015 4,748 7 View

Can anybody help with separation of genomic and viral episomal DNA?

I want to get pure genomic fraction and pure episomal fraction without cross-contamination. I want to use them in PCR. I am working with herpesvirus which has quite a large genome. Any suggestions?

09 April 2014 5,111 7 View

Does anyone have experience with separation monosaccharides (neutral and acid) on the Dionex columns?

Which column (CaerboPac MA1, PA1, PA100) is the best? Is it possible to detect neutral and acid monosacchairdes on the one column?

20 September 2013 4,314 1 View

How to increase tissue macrophages

Hello, I am currently in a desperate need of a group brainstorming and I thought I could use your help. I would like to develop an experimental model of human lung cancer and the aim is to test...

01 January 1970 164 1 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Database of European Football Players Salaries ?

Hello everyone, I am interested in writing about a dissertation about the wages of the football players of the 5 main leagues in Europe. I wanted to know if someone had an up to date data base...

17 February 2021 8,847 3 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

Where can I find FDI data by sector/industry for Asian countries like India and China for the last 30 years?

Common databanks like UNCTAD, OECD, WorldBank and ASEAN Website did not have the data for the last 30 years.

09 February 2021 4,701 4 View

What is India's InternationalTrade Policy during Pendamic? How do you find the policy as successful one?

During Covid-19 Pendamic Government are facing problem to maintain business. India is one of them so this question is perfect to be discussed here.

08 February 2021 2,725 3 View

I am struggling with the concept of preprint papers and wonder how people feel about them?

Specifically, how should we use them given they have not been peer-reviewed? Should we rely on the results reported in pre-prints for policy? I am really probing for thoughts on whether...

04 February 2021 3,175 15 View

What is the appropriate role of memorization in foreign language teaching?

Research in FLT has always been concerned with the role of memory in learning and the relationship between memorization and learning.

04 February 2021 2,202 5 View

Participation in questionnaire for PhD Thesis, any English, Chinese or Spanish speakers?

Hello! I am looking for Spanish, English and Chinese native speakers to participate in my final survey for my PhD thesis. https://claudiomastrangelo4.wixsite.com/phdlinguist1/research This is...

01 February 2021 4,177 10 View

Papers/studies on public policy and translation/implementation of global policy?

Hi all, I am looking for papers/books/studies related to changes in public policy, including theoretical papers, and on the translation/implementation of global policy onto the national level....

25 January 2021 2,242 7 View