Can nanoparticles access the brain after intranasal adminitration by endocytosis?

I found that functionalized nanoparticles (190 nm) can reach the brain easier than transferrin-functionalized nanoparticles (210 nm) after 30 minutes of an intranasal administration. Is it...

09 October 2018 5,719 1 View

Can I freeze at -20 C E coli suspension for posterior extraction of DNA without affect its integrity and purity?

I am using the QIAamp DNA Mini kit 250 from QiaGen for purify genomic DNA from E. coli. I have a biomass and I am looking for the best storage conditions because I am not going to perform the DNA...

06 August 2018 9,171 2 View

Can anyone identify this type of calcite crystal?

This is a SEM image taken after 7 days of carbonation of nanolime at 60-80%RH. Those crystals were found as pure calcite by XRD, which were fully oriented to {104}. Can anyone with experience on...

17 June 2018 2,446 27 View

Significant stored energy has been released means that likelihood of much larger earthquakes in the region has reduced now. " Anyone questions this??

"However, since two major earthquakes of magnitude 7 and above have occurred in a matter of 10 days in the region toward southern part of North American tectonic plate, significant stored energy...

08 September 2017 900 1 View

Any approach to accurately quantify low protein concentrations (10-500 ng/microL)?

27 October 2014 6,599 11 View

Does anyone know of research on business model innovation for mining industry towards sustainability?

I am exploring topics for my master thesis and I am very interested in business model innovation for sustainability applied to the mining sector since in countries such as Colombia, where I come...

01 February 2014 8,184 2 View

What does "MS-revised medium supplemented..." mean?

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12 September 2013 7,972 2 View

Is there a reliable/proven method for quantifying remyelination in grey matter?

Although not as highly myelinated as white matter, there is a significant amount of myelin in cortical gray matter. I'm having trouble finding a reliable method to quantify remyelination in grey...

02 March 2013 8,451 0 View

What's the poing on installing an AC/DC/AC converter to a SCIG wind turbine?

Besides for protection purposes, if you install the SCIG to the grid through a transformer, the system automatically achieves the balance between the mechanical and electromagnetic torque...

01 January 1970 8,497 6 View

Nice paper from Prof. Bastarrachea's Lab at UTSA: https://www.ncbi.nlm.nih.gov/pubmed/24553120

01 January 1970 913 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View