Can I freeze at -20 C E coli suspension for posterior extraction of DNA without affect its integrity and purity?

I am using the QIAamp DNA Mini kit 250 from QiaGen for purify genomic DNA from E. coli. I have a biomass and I am looking for the best storage conditions because I am not going to perform the DNA...

06 August 2018 9,171 2 View

Can anyone identify this type of calcite crystal?

This is a SEM image taken after 7 days of carbonation of nanolime at 60-80%RH. Those crystals were found as pure calcite by XRD, which were fully oriented to {104}. Can anyone with experience on...

17 June 2018 2,446 27 View

Significant stored energy has been released means that likelihood of much larger earthquakes in the region has reduced now. " Anyone questions this??

"However, since two major earthquakes of magnitude 7 and above have occurred in a matter of 10 days in the region toward southern part of North American tectonic plate, significant stored energy...

08 September 2017 900 1 View

Any approach to accurately quantify low protein concentrations (10-500 ng/microL)?

27 October 2014 6,599 11 View

Does anyone know of research on business model innovation for mining industry towards sustainability?

I am exploring topics for my master thesis and I am very interested in business model innovation for sustainability applied to the mining sector since in countries such as Colombia, where I come...

01 February 2014 8,184 2 View

What does "MS-revised medium supplemented..." mean?

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12 September 2013 7,972 2 View

Is there a reliable/proven method for quantifying remyelination in grey matter?

Although not as highly myelinated as white matter, there is a significant amount of myelin in cortical gray matter. I'm having trouble finding a reliable method to quantify remyelination in grey...

02 March 2013 8,451 0 View

What's the poing on installing an AC/DC/AC converter to a SCIG wind turbine?

Besides for protection purposes, if you install the SCIG to the grid through a transformer, the system automatically achieves the balance between the mechanical and electromagnetic torque...

01 January 1970 8,497 6 View

Nice paper from Prof. Bastarrachea's Lab at UTSA: https://www.ncbi.nlm.nih.gov/pubmed/24553120

01 January 1970 913 1 View

Interested in connecting this with our Cellulat system?

01 January 1970 7,948 0 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Can RNAscope be used to quantify the mRNA expression level of certain genes?

Hello, all, I want to explore the mRNA expression level of certain genes in the brain under physiological and pathological conditions. In previous studies, the researchers usually isolated the...

18 February 2021 1,518 1 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View