Hello
We are working on polysomal RNA isolation and interested in working on polysome profiling. Anybody having an experience or willing to collaborate on this, please send a message and we can discuss. Thanks
To do the spatial correlations between WRF model data and GPM rainfall data I need to re-grid the gpm data into 1km resolution please explain the process.
10 May 2024 3,413 0 View
I am performing fluorogenic assay with trypsin and fluorogenic assay Z-RR-AMC, my readings are decreasing with increasing time. What could be the reason for this strange results. Please...
01 April 2024 4,657 1 View
how easily is paper of editorial members accepted ?
22 December 2023 5,244 3 View
Hi , I have been trying to transduce my iPSCs with sgRNA , at MOI 5 and 10 and I have had very less efficiency . (3-4%) My Lentiviral supernatant is concentrated. Could some one provide some...
13 July 2023 1,557 4 View
It seems that some data are contradict to each other. The XRD assays shows the ZnO NPs have a size of X nm; where their DLS detection showed that these particles are in average of Y nm. Yet their...
03 June 2023 7,871 6 View
kindly provide the answer to my question sir/madam.
23 March 2023 5,370 4 View
Hi Has anyone generated Stable dCAS9 iPSC cells lines ? What are the methods that you have used? Have you tried selection using a flurophore which can be determined using FACs and sorted...
28 November 2022 6,711 0 View
Hi , I have been working with iPSC cell lines and hESCs for 6 years with Matrigel and mTESR1. I have recently started working with iPSC cells with vitronectin matrix and Stemflex media ....
18 November 2021 324 3 View
The published material mentions minimum dose as 1 mg. Is 0.5 estradiol effective in reducing hot flashes and if yes ,after how many days does response occur ,clinically ?
03 November 2021 8,385 3 View
I have purified a recombinant M0 AB(1-42) using a recent method from A Robust and Efficient Production and Purification Procedure... I have used 15mM NaOH as SEC buffer and also tried to keep...
22 July 2019 5,767 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?
03 August 2024 607 2 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View