Could someone share experience on the assay of Rat MCP-1 (Monocyte chemoattractant protein 1, also known as Chemokine Ligand 2 – a cytokine) in plasma/serum of rats using the RayBio MCP-1 ELISA kit. The manufacturers of the kit (RayBiotech Inc.) suggested a 500 to 10,000 fold dilution of the plasma/serum, and further stated that an investigator should determine the optimal dilution for his/her assay. Incidentally the contents of the test kit can only barely run the 12 strips of 8 wells (96 wells), and trying out varied dilutions across this wide range will almost use up our kit.
I will appreciate obtaining information on optimal dilutions commonly used by researchers who have experienced assaying for Rat MCP-1 using RayBiotech ELISA kit.
Thats quite a range of dilution.. What is the assay range of the kit?
The kit from Novatein Biosciences assay range is 15.6 - 1000 pg/ml and they suggest 5 - 10 fold dilution.
Thanks Mike. There is no defined assay range on the RayBio test kit manual, but the minimum detectable dose for the test kit was specified to be 15 pg/ml. However the concentrations of MCP-1 in the standards ranged from 24.67 pg/ml to 18,000 pg/ml.
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