What are the reasons for poor bandwidth in rapid tests?

I used covalent bonding to conjugate the antibody to the nanoparticle, but I get a weak band on the urine samples. What could be the reasons for this?

01 December 2020 4,202 2 View

Hi. My PCR result is showing nonspecific bands. I'm working on DNA extractions from dog blood to detect L. infatum infections by ITS rDNA gene?

I have changed my extraction method, changed my PCR master mix, changed my water. also I have modified my PCR program but nothing works. and when I got a single band and sent it for sequencing...

06 August 2019 4,371 2 View

How can I stop DNA annealing reaction?

14 December 2015 7,644 5 View

What would be the reason for lower extinction coefficient of fluorescent protein than the expected one?

I purified a mRuby2 protein from Bactria. the extinction coefficient of this protein is 113000 M-1cm-1. but my expressed mRuby2 protein has a extinction coefficient of 45000 M-1cm-1. what would be...

08 March 2015 6,375 12 View

How can I express a gene in pBAD vector?

19 February 2015 5,600 9 View

Who has the protocol honeybee (Apis mellifera) DNA extraction?

DNA extraction

15 December 2013 5,937 3 View

Anyone working with CLC Main Workbench program and knows how to add an annotation?

I have a sequence of my construct in CLC Sequence Viewer and I want to change the name of annotation and add another annotation to the construct sequence. Does anyone know how can I do...

11 July 2013 3,624 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

No mRNA increase in PCR after successful transfection in ARPE-19 cells ?

Hello, i am a bit curious about my results. I transfected the human cell Line ARPE-19 with a Plasmid containing a GFP tagged p53 (p53-GFP, Plasmid #11770). After 24h i checked under the...

18 February 2021 3,811 7 View