What are the reasons for poor bandwidth in rapid tests?

More Naghmeh Salmaniyan's questions See All

What is the main Method?

Dear all I am interested due to this project. Because i am writing such this subject for arid rangeland. It is useful for me knowing about method and processing. I will appreciate if you help...

26 February 2017 2,968 2 View

How to distinguish a monoallelic mutation from biallelic one when not dealing with a pure tumor sample (high contamination of wild type presents)?

My research is focused on Wilms tumor1 gene mutations in acute myeloid leukemia and we use bone marrow or peripheral blood samples; therefore, it is almost unlikely that I could be able to find a...

13 November 2016 2,378 2 View

How can I stop DNA annealing reaction?

I am annealing 37 DNA oligos together but i would like to stop it in certain time. i tried to add loading dye to stop it but it did not worked. Do you know how can i stop it?

14 December 2015 7,769 5 View

Why dose not a His-tag fused protein eluate during eluation step in FPLC even with > 2 M Imidazole concentration?

I am purifying a His-tag fused protein by Äkta instrument from GE Healthcare. The column in 1 ml HisTrap column. after loading the lysis to the column and washing by washing buffer, i elute the...

02 July 2015 8,651 12 View

What would be the reason for lower extinction coefficient of fluorescent protein than the expected one?

I purified a mRuby2 protein from Bactria. the extinction coefficient of this protein is 113000 M-1cm-1. but my expressed mRuby2 protein has a extinction coefficient of 45000 M-1cm-1. what would be...

08 March 2015 6,574 12 View

How can I express a gene in pBAD vector?

I have a fluorescent protein in a pBAD vector and I want to express and purify it. At first, I cultured a colony in 100 ml LB medium at 37 °C for over night. Then, I inoculated 25 ml from over...

19 February 2015 5,736 9 View

Why is the speed of scaffold lower than assembled DNA origami (6HB) in na agarose gel?

I assembled a DNA origami, a 6HB, and I run a scaffold and assembled 6HB in a agarose gel. In a gel, the band of scaffold is higher than 6HB band. actually, the molecular weight of 6HB is more...

27 August 2014 1,156 2 View

How to calculate signal to noise ratio in force data?

Some studies are related to this issue but I need the exact way to calculate in force data, which is collected by force plate in motion analysis lab.

12 March 2014 6,128 4 View

What is a reliable method to calculate knee adduction moment and proximal tibia shear force in ACL loading research?

I mention my method but I'm not sure, I couldn't find the exact method in the literature. Please have a look at my method and advise me if possible? Knee adduction moment: 1- After computing...

02 August 2013 1,404 2 View

How to optimize conditions for labeling of a hydrophobic peptide with Alexa Fluor 647-maleimide dye and Alexa Fluor 546 NHS ester?

Hydrophobic peptide (53%), 15 a.a.-long contains a single Cys-residue and Amine free, can be dissolved in DMF. I need to attach Alexa Fluor647-maleimide to the thiol group of the peptide and Alexa...

24 July 2013 8,874 7 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Ti6Al4V - Phase differentiation between alpha and alpha prime martensite?

Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...

30 July 2024 3,849 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.

23 July 2024 3,205 0 View

Michael G. Weller

How did you verify that your covalent immobilization worked as expected?

Naghmeh Salmaniyan

@ Michael G. Weller Because when I test with control, I get a better result, but when I put it in a urine sample, the bandage becomes weak.

I also checked the conjugation of the nanoparticle with the protein by electrophoresis.