Should one expect a single G1 peak or a wider S phase peak during FACS analysis of promidium iodide stained cells? I obtain population of S phase cells at T0 immediately after release from double thymidine block of 2mM Thymidine for 18 hrs and 17 hrs each.
Cells are arrested by intra-S phase checkpoint, due to imbalance in dNTP pool.
https://academic.oup.com/hmg/article/13/23/2937/625576
So, as for cell cycle phase, it must be in S phase checkpoint arrest. As for DNA content, it would be slightly more than G1 peak.
Shin-Ichiro Hiraga Thanks for the explanation. S-phase check point arrest means that cells do not enter S-phase at all. Still, how people show a typical G1 phase (2N) peak immediately after release from thymidine double block. For eg, in these papers:
Article Cell Synchronization by Double Thymidine Block
Fig AArticle Analysis of microRNA expression profiles during the cell cyc...
Fig 1Whereas, my thymidine double block cells are arrested in S-phase at T0 (see the attachment below)
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