How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why am I getting 'Infinity' and 'NaN' in my mixed-mode energy release values?

Hi everyone, I am currently running a mixed-mode simulation of a DCB using a user-defined cohesive zone model (UEL), however, the results I am getting don't make sense. I used the same UEL for...

24 February 2021 4,157 1 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

How to eradicate the deteriorated contact resistance in the electrical or electronic devices, like SCR or contactor' pin cable ?

Through years operation, some of the devices' pins connection gradually become worse and finally burn out due to the high temperature at the connection point. We can check out these risk points...

04 February 2021 7,401 5 View

How can I verify the design on the FPGA ZedBoard?

I have designed AXI Interconnect to connect 4 masters and 1 slave using SystemVerilog and Vivado Tool. The simulation stage of the design is working well. In the design, there are more I/O pins...

06 January 2021 8,487 2 View

I am trying model a pin connection in Abaqus, Can any one introduce a good reference?

I would like to model a real pin connection considering the "interaction" between pin and plate bodies. Drawing their base configuration in Autocad and then importing them as a sketch in Abaqus...

30 December 2020 2,268 1 View

Writing participant characteristics in method while being LGBTQI+ sensitive?

I apologize in advance if I come across as ignorant. I am trying my best to educate myself on LGBTQI+ community and how to write them in academic writing. I have a sample of X participants in a...

29 December 2020 9,011 3 View

Does anyone know how to go about staining both the apical and the basolateral side of a costar (pore size 3um) trans well?

I have cells seeded on both the apical and the basolateral side of a transwell. I would like to stain both sides to observe the cross talk between both sides. I've been looking for protocol, but...

07 December 2020 8,627 1 View

What is the type of connection between elements on ANSYS APDL after I merge their nodes together?

For the bridge shown below, the web is connected to both flanges, and the slab is connected to the parapet by meshing and merging the nodes, but what is the name of this connection? is it...

22 November 2020 2,288 5 View

Has anyone tried separating cis and trans isomers of fatty acids derivatized as their trimethylsilyl esters using N,O-BSTFA reagent on FAME columns?

I have not achieved separation of cis and trans fatty acids derivatized as trimethylsilyl esters using N,O -BSTFA on the DB-5 Column, but I prefer this derivatization method as it is rather...

18 November 2020 4,993 3 View

How to understand the spectral residual anomaly detection (SR-CNN)?

I was reading "Time-Series Anomaly Detection Service at Microsoft" (https://arxiv.org/pdf/1906.03821.pdf) in these days, and I got some problems for the programming part. The first picture...

17 November 2020 920 1 View