Dear all
I am looking for a method to stain blood vessels in the mouse retina in the UV spectrum while the retina is still alive and not fixated. The labelling protocol should not last too long (max 1-2 hours). Does anybody have a good idea or experience with mouse blood vessel markers in the UV spectrum?
Thank you
Dear Gerrit,
I have seen nice images of blood vessels in CNS people using Fluorescent dextran injected intravenously to label the vasculature. I think you may be able to inject Fluorescent dextran to the mice via jugular vein prior to dissection of retina, and that may able you to image or view vessels in whole-mount live retina.
Best wishes,
Refik
Dear Gerrit
As Refik suggests. I did it with zebrafish recently, where 2-4% (w/v) of 10 or 40 kDa with Alexa 488 was most useful. Thermo Fisher has a selection of different sizes a probes, se link below.
Best Kristian
https://www.thermofisher.com/dk/en/home/references/molecular-probes-the-handbook/tables/molecular-probes-selection-of-dextran-conjugates.html
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining