From literature and my own experience, to culture Ascochyta rabiei without supplementing the media with chickpea powder is not possible. I am curious to know what's the active ingredient in chickpea powder which is actually required to nurture its culture and can we supplement it chemically instead of raw chickpea powder or chickpea extract?
Dear @Amina Ilyas
Phytoalexins accumulate in plants or cell cultures only transiently, because they are degraded or polymerized by extracellular peroxidases. The degradation of phytoalexins may be important for the development of particular plant diseases. For example, Ascochyta rabie causes the oxidation and reduction of the phytoalexins (medicarpin and maackiain) in chickpeas. Thus a knowledge of phytoalexin catabolism will underpin the development of compounds resistant to degradation by pathogens. I have attached two pdfs. Please check whether both are useful to you.
Best wishes, AKC
Article Ascochyta blight: Isolation, characterization and developmen...
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