can anyone suggest about in vitro assay of anti-PD-L1? which assay can be performed to check anti-PD-L1 function in vitro?
its amazing question although now I don't have the answer but I thanks you for ask like this questionh
How to qualitative data analysis from open questionnaires to a positive outcome
03 June 2024 9,829 3 View
Can anyone please share such questionaires for a project study please
29 April 2024 5,946 5 View
Effectiveness of coaching is possible . Can anyone enlighten please on this subject matter ,Required for a project.
29 April 2024 8,536 2 View
Dear all I am trying to run RF DIFFUSION tool locally. While running the script inference.py I am getting error "NVTX functions not installed. Are you sure you have CUDA build? " I have latest...
20 March 2024 2,384 1 View
I was trying to do RRDE experiment using Gamry. I appiled 1.2 V vs Ag/AgCl at the ring and 0 to -0.65 V vs Ag/AgCl at disk . The potential at disk keep stuk at around 1 V and does not go over the...
19 March 2024 2,230 0 View
I am trying to isolate microbial DNA from soil samples stored at -80ºC ( one year old stored sample) using Nucleospin soil kit (Make: Macherey Nagel) but unable to get the DNA from stored samples...
09 August 2023 3,777 2 View
desorption mechanism for both arsenic species (AsIII and V) using NaOH as a desorbing solution
30 July 2022 4,687 0 View
For covid19 diagnosis TaqPath kit is having ABY n JUN dye... But in our lab Biorad Cx96 machine is available where ABY n JUN dye are not available. How can we use the Biorad machine for covid19...
22 July 2020 2,364 2 View
For calculating Q-index for phytoplankton functional group can we consider only the water depth or should we also consider the area of the aquatic body?
24 May 2019 2,329 6 View
I am doing a photo-catalytic experiment using Cotton fabric-TiO2 NPs composite material with UV light assists to degrade Rhodamine-B dye. During the degradation process a hypsochromic shift is...
01 July 2018 1,036 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View