JUN and ABY dye are not available in Biorad machine. Can any one tell me which dye (present in biorad machine) wavelength is similar with JUN n ABY ??

For covid19 diagnosis TaqPath kit is having ABY n JUN dye... But in our lab Biorad Cx96 machine is available where ABY n JUN dye are not available. How can we use the Biorad machine for covid19...

22 July 2020 1,978 2 View

Is it possible to find the sensitivity of RMSD and RMSF in MD simulation through gromacs ?

Is it possible to find the regions of the protein structures most susceptible to temperature impact in terms of RMSD and RMSF , during MD simulation ?

06 May 2018 8,855 3 View

Can anyone suggest me where and how to apply for post-doc as my work is on utilization of Water Hyacinth

26 July 2017 3,040 3 View

What should be the ideal settings for HSP length cut-off and HSP-Hit coverage for running Blast in Blast2Go?

I have to analyze PacBio transcriptome data with four bin sizes: 1-2 Kb, 2-3 Kb, 3-6 Kb and 5-10 Kb. I can understand that the HSP length cut-off and HSP-Hit coverage settings must be customized...

26 July 2017 1,346 1 View

Can anyone identify the two plant species

The plants were collected from Barak Valley Assam

24 May 2017 9,971 6 View

By which means can the porosity be determined in a vegetal fibre ?

We develop a new mechanical characterization test for vegetal fibres. We need to know the apparent density of them. We can know easily the real density by pycnometry. We want to determine the...

12 April 2016 7,592 8 View

What are the advantages and the drawbacks of AFM and nanoindentation ?

I need to measure the mechanical properties of a composite material on its surface (so there are several material phases). Which method is better between AFM and nanoindentation ?

03 September 2013 2,030 9 View

Sample making for ELISA

At first I treated interferon beta protein to the tumor bearing mouse then I collected the tumor and blood from the mouse. I already made the tumor sample with PBS and collected serum from blood....

01 January 1970 7,883 4 View

ELISA sample preparation

I want to check interferon beta protein concentration in mouse tissue sample like heart, liver, lung and kidney. Can anyone please help how can I make the sample from the tissue for ELISA?

01 January 1970 1,848 3 View

ELISA standard curve

when we calculate the concentration of unknown samples based on standard curve, which range of data can be used? Lowest and highest range?

01 January 1970 2,748 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View