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The method of purification of the antigen plays a key role, apart from that the temperature susceptibility, the nature of the protein molecule(antigen) will be my opinion Sir.
I have labeled my replicating bacterial DNA with 5-Ethynyl-2'-deoxyuridine (EdU) and want to click it with biotin-PEG-3-azide followed by enrichment with magnetic streptavidin beads. So far, I...
06 February 2024 5,381 0 View
I have prepared selenium nanoparticles and added polymer as a stabilizing agent, practically, the solution was clear for months and no visible precipitation was observed, however, the zeta...
27 January 2024 6,107 4 View
I plan to research "Construction Solid Waste Management using Digital Twin Technology." However, I'm still a bit confused about narrowing down my research area. A few key areas come to mind, such...
07 June 2023 7,972 0 View
Who else get frequent emails from Social Sciences (SS) e-ІЅЅN:2326-988X to join them as Editorial member? What are your experiences?
23 February 2023 7,100 3 View
I have to do proximal mapping of a complex vector p onto the surface of M dimensional complex sphere, if someone can suggest to me some literature and toolboxes from which I can get some basic...
04 September 2022 8,544 0 View
I have L16 for Machining and some researchers do RSM after GRA using GRG data. How we can do that? Thanks
02 August 2022 6,196 1 View
and for Total Variation optimization how to frame the objective function having L1 norm function then what is the alternative way of imposing Total variational regularizer .
17 February 2022 5,224 4 View
While working on our own research paper, we need to cite the sources. Should we cite primary or scondary source.
11 February 2022 6,027 5 View
I want to extract the T6P (Tre6P) from plant leaves. We have LC-MS-QTOF in our lab instead of Q1 or Q3, so could anyone please help guide me about the method used for the T6P metabolite content...
15 October 2021 3,500 3 View
Particularly, if it is also software integrated.
12 September 2021 8,316 5 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?
02 July 2024 9,411 2 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hello everyone, I usually use the saphenous vein to collect blood from HIV-infected mice (for viral load, CD4/CD8, etc.). However, I would like to know what methods other researchers are using to...
27 June 2024 7,707 2 View