Asim Shaghil

6 Questions 16 Answers 0 Followers

Questions related from Asim Shaghil

If made 400L of MEM 199 medium and added 8 L of extra water?

I made 400 L of MEM 199 medium (sigma powder cat M0393 Medium 199 ) and added extra 8 L of water. Some people saying that i over diluted this media and it wont be good. Please give your opinion....

21 October 2016 6,552 3 View

Good Review article on Gene silencing?

I am looking for detail review articles on Gene silencing. Please let me know if recently gone through some? Thanks

16 January 2016 9,138 4 View

How to coat porcine saliva on Maxisorp 96 well plate?

I am trying to bind porcine saliva (2.3 mg/ml) on MaxiSorp plate. Any one have idea how to detect that protein really bind in the well. Please recommend me a method that can detect the...

29 September 2015 1,603 1 View

Looking for a good methodology for the protein extraction from porcine oral saliva for MALDI-tof analysis in bacterial adherence studies?

I wanted to experience a good methodology for the protein extraction from porcine saliva for MALDI-of analysis of bacterial adherence studies. I am aware of using the solubilization buffer ( 7M...

22 June 2015 8,682 1 View

In the biotech industry regarding stability of viral antigen, what are the factors that can effect the stability of the product?

23 February 2013 7,152 1 View

CMV Seed prepration

I want to make CMV seed from MRC-5 and I want to separate the CMV infected MRC-5 on day 4 of infection. Has anyone ever tried to separate the CMV infected cells by density gradient centrifugation?...

11 January 2013 2,254 8 View