@Onyeka, add together the live and dead cell count to obtain a total cell count. Then divide the live cell count by the total cell count to calculate the percentage viability.
Срок годности как и срок хранения определяется по показателям жизнеспособности по количеству выросших колоний, расчет либо по методу Коха, либо методом Miles&Misra
@J.C Tarafdar, thanks for your response but I wish to ask, how can I calculate the dead cells when I used the plating method for counting.
@ Onyeka, dead cells are blue because trypan blue can only permeate damaged cell membranes. Using the differential cell counter, count normal cells on one side, and blue cells on the other side. You can calculate cell viability by using the following formula: 100*(live cells)/(dead cells + live cells).
J. C. Tarafdar
Please note - this is in context of a microbial mass on solid media (assume agar plates). The poster is not about to stain the entire microbial mass - even if it was possible. Further - with uncountably great number of cells, a change within % levels is irrelevant and would not be statistically significant. Colonies always include both living and unculturable cells.
@J.C Tarafdar Am I meant to stain? My work was actually to check the shelf life of the Microbes in the powder media for months. So at each month after serial dilution, I inoculate on agar plate and then do my plate count. So maybe after 3months, how do I get %viability
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