In RFLP gel run, the ladder ran faster than sample, can anyone suggest what could be the error?

21 July 2021 1 1K Report

Hello,

I ran RFLP gel where I noticed that the ladder ran faster than the sample. When the ladder dye crossed the plate, I took the gel out and visualized. Now, I don't understand why this happened. I followed our standardized protocol. I ran this gel for other samples before and it came out well.

Please suggest what might have went wrong, Is it with gel composition or something else.

I diluted the ladder with loading dye and water (as per the protocol) and kept at 4degree a day before. Can this be an issue? Please suggest.

Riaz A. Khan

Dilution seems to be the spoiler.

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