Attached I send two documents in PDF with photographs of the sample, stained with MGG, another with Pap. Final magnification of 400x.
well, to me these cells seem to be either reactive mesothelial cells or lung adenocarcinoma cells.... lung adenocarcinoma cells are notorious in mimicking reactive mesothelial cells...??? i would advice TTF-1 to differentiate between them...?? all the best.
these cells are the size of lymphocytes with very dense nuclei, I think they could be apoptotic cells, perhaps due to therapy?
you can try confirm apoptotic cells by immunocytochemical detection of c-caspase 3?
check this article http://www.thelancet.com/journals/lanres/article/PIIS2213-2600(14)70050-5/fulltext?rss=yes , where similar cells are shown
possibility of plasma cells should also be kept in mind..?? as their shape resembles more to plasma cells.
Dear Dr. Banyameen Iqbal, thanks for your answers. This can't be mesothelial cells because is a sputum and is too small for ADC. Best wishes, Carla
Dear Irena, how are you? Me and Rúben have the same opinion than you. We made ICC in cytospins and only CD45 was positive for some cells. We are waiting for more sample to do a cell-block for ICC (caspase). Thanks for your help and this article. Warm regards, Carla
Hi Carla, you can also send us a cytospin for caspase staining, we already did a study on a cell line with apoptosis induction so we are sure that you can reliable detect apoptosis by caspase on methanol and PEG protected cytospins!
From their size and plasmazytoid Features, These cells have to be interpreted as plasma cells first. Since plasma cells do not express CD45, I advice to perform immunocytochemistry for CD138, CD45 as well as pancytokeratin.
Dear Irena, that's great! Thank you so much for your help, Ruben is on holidays but when he come back we'll talk to you about this :) Warm regards, Carla
Dear Dr. Bart Vrugt, thanks for your answer. That's another good possibility try CD138, thanks :) Best wishes, Carla
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