I want to transfect neuronal cells with my gene of interest. The lab used AAV vectors containing hrGFP. Hence want to know how it is better than the other variants.
still I would stick to mGL, discovery of Dr Campbell
Article mGreenLantern: a bright monomeric fluorescent protein with r...
https://www.agilent.com/en/vitality-hrgfp-mammalian-expression-vectors-details
Hello Rutuja Pendharkar
hrGFP fluoresces brighter in mammalian cells and is less toxic than GFP/eGFP, making it easier to use for mammalian expression experiments. It has a higher extinction coefficient and is more resistant to pH induced conformational changes as well as to solvents, detergents, and proteases.
However, in the paper attached below, use of hrGFP instead of GFP did not result in a larger proportion of transfected cells expressing fluorescence for extended period of time after transfection. Therefore, hrGFP does not appear to offer a significant advantage over GFP in transfection experiments.
Article Time Course of Fluorescence Intensity and Protein Expression...
Best.
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