In order to transfect AAV based vectors in neurons why hrGFP is a better choice that GFP or eGFP?

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Adam Figarski

still I would stick to mGL, discovery of Dr Campbell

Article mGreenLantern: a bright monomeric fluorescent protein with r...

https://www.agilent.com/en/vitality-hrgfp-mammalian-expression-vectors-details

Malcolm Nobre

Hello Rutuja Pendharkar

hrGFP fluoresces brighter in mammalian cells and is less toxic than GFP/eGFP, making it easier to use for mammalian expression experiments. It has a higher extinction coefficient and is more resistant to pH induced conformational changes as well as to solvents, detergents, and proteases.

However, in the paper attached below, use of hrGFP instead of GFP did not result in a larger proportion of transfected cells expressing fluorescence for extended period of time after transfection. Therefore, hrGFP does not appear to offer a significant advantage over GFP in transfection experiments.

Article Time Course of Fluorescence Intensity and Protein Expression...

Best.