Dear Muhammad Hammad Rasool ice can only form at 0 C whereas hydrate formation is highly dependent on HLVE conditions which varied with the presence of different gases and pressure conditions.

As there is no stirrer inside the micro DSC cell (limited mass transfer), you need high degree of subcooling that will result in ice formation to facilitate the hydrate nucleation. However, on heating - the ice will melt before the hydrates (as obvious).

Two types of experiments can be performed using mico DSC: ramping and isothermal tests. A disadvantage of the temperature ramping

method is that the temperature conditions are suitable for forming

both ice and hydrate. As the ramping continues to lower temperatures, more ice tends to form. This is undesirable since we want both water

and inhibitor to be available for hydrate formation and inhibition.

Some inhibitors may also inhibit ice formation (e.g., antifreeze

proteins), and inhibitors that bind to the ice would then not be

available for hydrate inhibition. However, since we can identify the

approximate nucleation temperature during ramping experiments, it is

possible to reduce the temperature quickly to slightly above the

hydrate nucleation temperature and then remain at that temperature

for a longer time. In this method, the strength of the inhibitor with

respect to hydrate nucleation inhibition is expressed in terms hydrate

nucleation hold time at given constant temperature (isothermal run).

As Tariq suggested the system is mass transfer limited, one has to go at very low temperature where ice and hydrate both can form. It is difficult to distinguish ice and hydrate exothermic peaks (during formation), a big ice peak might be a combination of both. Sometimes we can see only one exothermic peak during formation. Only the endothermic peak will give you a better idea based on the melting temperature. Even you can observe hydrate formation after ice melting. (refer to our paper for better understanding)

If you want to understand the induction time through DSC then perform experiments above zero temperature.

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@Asheesh , I am using 20 - -20 C for cooling at 114 bar. In my experimentation, using mucro- DSC, in an exothermic scenario, two peaks are formed, obviously the smaller one for hydrates but in some scenarios under the same set of condition only one big peak is formed, as in no peak for hydrates. How do you see it?