Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can RNApolymerase bind to RNA?

Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?

14 December 2020 2,708 5 View

What are the methods used to amplify the voltage received from two electrodes used in a micro channel to count micro and nano particles?

i'm recently on counting of micro & nano particle by methods like:impedance spectroscopy And I want to get a stronger voltage from my electrodes Who can give me a solution in this matter?

30 June 2020 6,963 8 View

Reinforcement learning toolbox?

I am looking for Matlab Reinforcement learning Toolbox, Can anybody help me? I will b so thankful.

31 May 2020 3,767 3 View

I want to compare the difference of immune system in patient with mild and sever symptom.who should i define the case and control group?

Is it enough to compare people who needed to be hospitalized with people who did not? , or should the age and duration of the illness be considered?

26 May 2020 2,829 2 View

Why the covid-virus infection is very rare in children compare to young and adult people?

As we know the immune system in both children and adult is impaired. The question is that why the covid-virus infection is very rare in children compare to young and adult people

03 April 2020 9,934 11 View

Which module is better for parallelism numpy functions in Python?

Which you recommend?Numba,Joblib,Dask and etc.Why?

20 March 2020 4,529 4 View

Can anyone advise if there is any company that sells a specific antibody for the HLA DRB1*1501 allele?

Hi everyone, I would appreciate if someone can give me some advise regarding Can anyone advise if there is any company with a specific antibody against HLA DRB1*1501 allele (DR 15 allele HLA...

29 November 2019 1,923 2 View

In Rev manager with continuous data, what number do we have to put in the Mean part while we have two pre and posttest means in the main articles?

I am working with Rev manager 5.3, doing a meta-analysis on 8 studies that each compare 2 psychological treatments and found no difference between them.

25 September 2019 6,804 1 View

Why do we have differences in numerical and experimental models in the damaged plastic model?

Considering the start of the cracks in the middle of the sample and the acts boundary conditions on both sides of the strain stress curve for composite It is linearly drawn up to the point of...

13 February 2019 2,176 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to determine a priori sample size for t-test with unequal means?

I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...

24 February 2021 8,289 3 View