Does increasing the number of individuals by copy-paste of the same indvs to perform GWAS affect the results?

I am trying to perform a genome-wide association on mitogenome variants (obtained from GATK-Mutect2) vs. phenotypes by using GAPIT. The number of individuals is seven and GAPIT function does not...

14 December 2020 8,853 3 View

How can I make Isight interact Solidworks with Abaqus?

Hello Everyone, I need to optimize a simple beam natural frequency in Abaqus with Isight. I know in abaqus there is parametric tool but for more complex and further study I want to use Catia or...

25 October 2020 7,007 3 View

Second wave of COVID-19 cases is bigger than the first one in Europe. Do you believe it?

29 September 2020, NewScientist In Europe, the long-predicted second wave of the coronavirus pandemic is now well under way. Several countries are reporting more daily covid-19 cases than they...

11 October 2020 461 5 View

Buffer solution 13 is prepared from a strong base sodium hydroxide and potassium chloride salt ..???

Buffer solution 13 is prepared from a strong base sodium hydroxide and potassium chloride salt .. While the definition of buffer is contain from weak base or weak acid with their salts .. So why...

03 September 2020 7,802 7 View

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12 August 2020 7,970 42 View

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12 August 2020 259 9 View

"Second COVID-19 wave could be twice as big as the first without effective test, trace, isolating strategy." Can you believe it?

Second COVID-19 wave twice as big as the first without effective test, trace, isolating strategy, says new modelling study Reopening schools fully in September without an effective test, trace...

05 August 2020 348 5 View

Only 2.5% of Italians have Covid-19 antibodies, government study shows. Is it good for herd immunity?

4 August, CNN Authorities in Italy have found that only 2.5% of Italians, or 1,482,000 people, have Covid-19 antibodies despite it being one of the countries hardest hit by coronavirus in...

03 August 2020 2,649 20 View

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27 July 2020 4,032 92 View

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Public health officials need to "stick with the science," even in a political atmosphere, Fauci says A pandemic may intensify the divisiveness that already exists in the country, but scientists...

24 July 2020 9,262 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can I measure absorbance efficiency with wavelength in a spectrophotometer?

I measure the wavelength of material in a spectrophotometer and it peaks at 275 and its absorbance is 100. I mix it with an adsorbent and it adsorbs the first material, but when I measure the...

16 February 2021 7,515 5 View

CAT assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!

11 February 2021 7,273 3 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

What dilution do I use for my GNP solution UV-Vis and DLS characterization?

I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...

26 January 2021 530 5 View

How to calculate DNA concentration of a sample with high concentration buffer?

I want to calculate the concentration of DNA in an unknown sample. But it's buffer has a high concentration and our nanodrop don't accept that as a blank of dsDNA. Thus I dilute the buffer with...

23 January 2021 9,440 2 View

Anyone know how to dilute strong hydrogel structure?

I am working on PVA-GMA hydrogel that is a really strong polymeric structure like rubber. Because I am in the middle of analysis, it is difficult to dissolve the gel using a common solvent. I...

20 January 2021 3,501 2 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View