In Fura-2 AM assay, what will happen if I didn't wash the cell thoroughly after the loading of dye?

12 October 2015 1 870 Report

What's the property of the uncatalyzed fura-2 AM? The spectrum and the property of binding of calcium.

Thank you for concerning.

The AM ester form is essentially non-fluorescent and does not have an appreciable affinity for calcium. The main problem that you would encounter is that the cell will continue to accumulate Fura-2. However, I do not anticipate that this would be a serious problem for a ratiometric dye, but you should take the precaution of recording the Ca2+ insensitive fluorescence more frequently than would otherwise be necessary.

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