Sui Chen

3 Questions 2 Answers 0 Followers

Questions related from Sui Chen

Is that OK if I keep the buffer solely buffered by HEPES in the 5% CO2 incubator?

Buffer: 145mM NaCl, 5mM KCl, 1mM MgSO4, 10mM D-Glucose and 10mM HEPES, 2mM CaCl2, 2.5mM Probenecid. I just found from online someone said buffer solely buffered by HEPES should not be kept in CO2...

11 December 2015 8,475 3 View

Can I do fura-2 assay in DMEM or similar medium instead of HBSS?

Why most people use HBSS to do calcium assay? I am now using HBSS to do this but I have to incubate the cell for more than 6h and I found that the cells were not good after such long incubation...

24 November 2015 1,402 3 View

In Fura-2 AM assay, what will happen if I didn't wash the cell thoroughly after the loading of dye?

What's the property of the uncatalyzed fura-2 AM? The spectrum and the property of binding of calcium. Thank you for concerning.

12 October 2015 869 1 View