Sui Chen

4 Questions 3 Answers 0 Followers

Questions related from Sui Chen

Is that OK if I keep the buffer solely buffered by HEPES in the 5% CO2 incubator?

Buffer: 145mM NaCl, 5mM KCl, 1mM MgSO4, 10mM D-Glucose and 10mM HEPES, 2mM CaCl2, 2.5mM Probenecid. I just found from online someone said buffer solely buffered by HEPES should not be kept in CO2...

11 December 2015 8,500 3 View

Can I do fura-2 assay in DMEM or similar medium instead of HBSS?

Why most people use HBSS to do calcium assay? I am now using HBSS to do this but I have to incubate the cell for more than 6h and I found that the cells were not good after such long incubation...

24 November 2015 1,424 3 View

In Fura-2 AM assay, what will happen if I didn't wash the cell thoroughly after the loading of dye?

What's the property of the uncatalyzed fura-2 AM? The spectrum and the property of binding of calcium. Thank you for concerning.

12 October 2015 893 1 View

Why do my CHO-K1 cells grow so fast?

I splitted my cell 1in 8 from almost 100% confluence and then they reached 90% confluence 24h later. Is that normal for CHO-K1 cells? I used advanced DMEM/F12 with 10% FBS, 1% L-Glu and 1% PS as...

12 October 2015 1,726 3 View