I am studying solid-liquid phase transition of lipids as function of temperature. In DSC micrograph, which points, either onset point of melting or melting peak, would indicate melting point. For example in case of lauric acid, the difference between two remains around 3-5 C i.e. melting onset at 43 C and peak at 47 C. Literature reports lauric acid melting point to be around 43.5 C. Are there any other factor that can be helpful regarding solid-lipid phase transition of lipids? Thank you
Thank you Asif, I am getting endothermic peaks. But in case of a certain peak, whether it is onset point or the peak which truly describes melting point.
Your DSC instrument was calibrated with a reference standard such as Indium to correct for temperature. When the instrument performs this operation it uses the melting peak onset temperature to set the calibration. Therefore, when analyzing fatty acids or other materials, the MP should be taken at the onset temperature, not the peak maximum. Also note that the onset is measured at the intersection of lines drawn tangent to the baseline and the inflection point on the fronting side of the peak.
Thank you very much GWH Hohne and Robert Dunn, you solved my problem. I am an end user of such facilities and did not knew principles/science underlying it.
Thanks again
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