Two virus strains were used, say A and B, and serum raised from each virus were tested against themselves (homologous) and the other strain (heterologous). The ND50 titre was calculated by Karber formula. The homologous titres were normalized to 100 and the heterologous titre was calculated accordingly. The titre of serum A against virus B after normalization is 40 and Serum B against virus A is 120. Now my question is, after normalization, at what percentage should I call the strain as cross neutralizing? Like in this case, serum B is showing higher heterologous neutralization than serum A, so it is definitely cross neutralizing. But in case of serum A, what should be my threshold to call serum A as cross neutralizing? Please provide reference.
Hi Sonal, I am not sure if cross-neutralization is a strictly qualitative feature. To me it is more of a quantitative thing, where an antibody can be strongly cross-neutralizing to one strain, while weakly cross-neutralizing to the other. If you agree with this, your serum A is weakly cross-neutralizing to strain B.
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