In quantitative real time PCR, will the machine calculate amount of RNA copies in the sample if the ct value lies beyond the standard range?

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Yoke Kqueen Cheah

Dear Sonal,

It depends on the initial information you input to generate the standard curve.

Regards

Timmy Richardo

Yes, I agree with Yoke, the quantification of RNA in the qRT-PCR will be based on the formula which you need to input before running the PCR. Your RNA copies will be reflected from the Standard curve.

Thanks