Pradip Karmakar

4 Questions 6 Answers 0 Followers

Questions related from Pradip Karmakar

In ChIP, why am I always getting "DNA too low to be detected" after IP?

I am using HEK293 cell line and following this protocol from...

10 February 2025 7,394 1 View

How do you separate the nucleus before performing sonication on mammalian cells?

pllease, share your protocol with me if you work on HEK or Hela cells.

20 November 2024 4,741 2 View

In Chip-seq, what will be the impact of adding proteinase K before removing the magnetic beads?

I was following this chip-seq protocol https://www.cellsignal.com/products/chip-kits-reagents/plus-sonication-chromatin-ip-kit/56383 what will be the consequence if on the following state "VI....

21 July 2024 5,675 0 View

How do you prevent HEK293 cells from detaching during PBS washes?

Do you use collagen solution or L-Lysine solution?

01 January 1970 624 3 View