In case of Nanodrop DNA conc. and quality check for QIAGEN kit, what would be the blank solution?

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Satyendra Mondal

The blank solution should be the elution buffer in which you eluted your DNA samples. If you eluted in distilled water, than use the same for the blank.

Paul Rutland

A high od260/280 probably means that you have rna present and Satyendra Mondal is right use the elution buffer supplied with the kit for a blank

Monisha Mitra

The blank would be the solution in which you have eluted the DNA. If you have used the kit elution buffer then blank your nanodrop with this one. Mostly we elute our DNA in prewarmed TE. So we blank it with TE