Currently I use CD95hi CD38lo as my gates on flow cytometry to elucidate the germinal centre cells (after gating on IgD neg, CD19 pos cells)
Hi Haley,
As long as you keep using the CD38lo phenotype on top of CD95 or GL7, I don't think using one or the other would make any difference. However, both GL7 and CD95 are B cell activation markers early in T-dependent responses so they label a population of activated pre-GC B cells that are distinct from GC (but are CD38 positive). Justin Taylor and Mark Jenkins had a nice paper about those cells in JEM a few years ago.
Best,
Pierre
Thank you!!! Would you have any advice on the use of CD80 and CD86 as indicators of activation? From what I gather a lot of people isolate splenocytes and stimulate in culture to capture this population, I would rather isolate splenocytes post-immunisation and directly check them on FLOW for these activation markers. I am having a hard time trying to find an article which says what time point is best to identify these cells. Most will look post 24-48 hours in culture stimulation, but not directly ex vivo. Thanks Pierre.
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