If you have any ideas concerning the right concentrations to be use, may be you should make a screening test using differents concentrations from 1-10 microgram/mL of extract or from 10-100.

Initially you can begin with 100 mcg/ml. If you get a positive anti-proliferative activity, you can take different concentrations ranging from 1 to 100 mcg/ml to calculate IC50 value for the extract.

Thank you for your proposals